TABLE 1.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Relevant characteristics | Reference(s) or source |
|---|---|---|
| Strains | ||
| E. coli S17-1 (λpir) | recA::RP4-2-Tc::Mu Km::Tn7 λpir | 5, 13, 35 |
| C. crescentus | ||
| CB15N | Synchronizable mutant of CB15; syn-1000 (= NA1000) | 9 |
| LS800 | CB15N recA526 with pKM3001 | 24, 28 |
| SGC103 | CB15N recA526 katG+ katG::lacZ translational fusion with pKM3001 | 38 |
| SGC109 | CB15N recA526 katG+ katG::lacZ translational fusion | 38 |
| SGC111 | CB15N katG17::(Spcr/Smr) katG null | 38 |
| SGC125 | SGC109 random Tn5 (Spcr/Smr); control Tn5 insertion strain | This study |
| SGC126 | SGC109 skgB::Tn5 | This study |
| SGC127 | SGC109 skgA::Tn5 | This study |
| SGC130 | CB15N recA526 skgA+ skgA144::lacZ translational fusion | This study |
| Plasmids | ||
| pJBZ 282 | Kmr; ori ColE1; for construction of translational lacZ fusions | 2 |
| pUT-mini-Tn5 Spc/Sm | Apr Spcr Smr; ori R6K; mob RP4 tnp* of Tn5-IS50R | 5 |