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. 2023 Jul 10;14:1223312. doi: 10.3389/fendo.2023.1223312

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Copyright © 2023 Sekhar, Codreanu, Williams, Rathmell, Rathmell, McLean, Sherrod and Baregamian

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Parathyroid Hormone (PTH) Secretion Analysis of Previously Cryopreserved Parathyroid PDOs. (A) Cryopreserved parathyroid PDOs were re-cultured and images captured with bright field microscopy (20x magnification). (B) Determination of parathyroid hormone (PTH) in the supernatants of re-cultured, previously cryopreserved parathyroid PDOs collected at 2 and 24 hours after thawing by ELISA. There is significant elevation of PTH levels at 24 hours of culture compared to 2-hour culture (p ≤ 0.0001).