Figure 1.

Study design. Recipient mice were fed chow or high-fat diet (HFD) for 14 weeks. After the first 10 weeks, fecal virome transplants (FVT) were administered via oral gavage every weekday for 4 weeks (red vertical bars) and feces were collected at regular intervals (dots). FVT were prepared in parallel from a separate group of donor mice on chow or high-fat diet. Gavage treatments consisted of chow-derived and HFD-derived viromes, as well as PBS controls. The study was conducted in 2 experimental replicates (i.e., trials). Trial 1 consisted of 4 mice per cage and 1 cage per treatment. Trial 2 consisted of 3 mice per cage and 3 cages per treatment. In Trial 2, we collected feces from 2 additional pre-gavage timepoints (purple dots). Five mice were omitted from the study due to death (one in Trial 1 HFD : PBS, one in Trial 2 HFD : PBS) or aggression toward other mice (one in each Trial 2 HFD : Chow, Chow : PBS, and Chow : HFD).