FIG. 3.
Size exclusion chromatography of an enriched diapophytoene desaturase fraction. (A) Elution profile at 280 nm. (B) Corresponding specific activities (milligrams of product formation/milligrams of diapophytoene desaturase) of the fractions. (C) Protein separation on a denaturating polyacrylamide gel of 500 μl of each fraction after concentration. Lane M, marker proteins; lane A, aliquot of 5 μl of initial partially purified protein preparation prior to size exclusion chromatography.