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. 1999 Oct;181(19):6184–6187. doi: 10.1128/jb.181.19.6184-6187.1999

FIG. 3.

FIG. 3

Size exclusion chromatography of an enriched diapophytoene desaturase fraction. (A) Elution profile at 280 nm. (B) Corresponding specific activities (milligrams of product formation/milligrams of diapophytoene desaturase) of the fractions. (C) Protein separation on a denaturating polyacrylamide gel of 500 μl of each fraction after concentration. Lane M, marker proteins; lane A, aliquot of 5 μl of initial partially purified protein preparation prior to size exclusion chromatography.