TABLE 1.
Purification of the diapophytoene desaturase from S. aureus
| Step | Vol (ml) | Amt (mg) of:
|
Relative CrtN content (%) | Sp act (ng/mg · h) | Recovery (%) | |
|---|---|---|---|---|---|---|
| Protein | CrtN protein | |||||
| Cells | 5 | 28 | 8 | 28.8 | 100 | |
| Membranes | 5 | 11 | 5.6 | 51 | 70 | |
| Supernatant | 5 | 16 | 2.56 | 18.8 | 33.2 | 32 |
| Flowthrough | 5 | 14 | 1.86 | 13.3 | 23.3 | |
| Purified CrtN | 3.5 | 0.056 | 0.054 | 97.2 | 300 | 0.7 |