FIGURE 3.

Scatter dot pots demonstrating platelet activation measured as number of P‐selectin positive platelets (%) or response to agonists, ADP or thrombin, measured as fold change in median fluorescence intensity (MFI FC log ₁₀), by flow cytometry in 9 cats treated with 7 days of rivaroxaban (RIVA), clopidogrel (CLOP) or dual antithrombotic treatment (DAT). (A) Only cats treated with DAT had significantly lower number of P‐selectin‐positive (%) resting (unstimulated) platelets compared to single agent treatment with RIVA or CLOP. (B) When platelets were treated with 20 μM ADP, CLOP or DAT significantly modulated response to ADP, while 7 days of RIVA treatment resulted in an increase response to ADP, which were significantly higher on D7 compared to CLOP or DAT. (C) Only DAT modulated platelet response to 0.005 U/mL thrombin, as shown by decreased percentage of P‐selectin‐positive platelets, and MFI FC from D0 to D7. Response to thrombin was significantly higher on D7 in cats treated with RIVA alone. *P < .05; **P < .005; ***P < .001; ****P < .0005.