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Effects of mutations in the −59 to −35 region of P-dapA on promoter activity. The arrows mark the inverted repeat. Replacement bases are indicated in bold face. Transcriptional activity of the mutated fragments was evaluated in the vector pET2. TS marks the transcription start (underlined A). CAT activity is expressed relative to that measured with the WT promoter. Standard deviations (SD) from three independent measurements are shown.
