FIG. 3.

Effects of mutations in the −10 region of P-dapA on promoter activity. Replacement bases are indicated in boldface. CAT activity measured by using the transcriptional fusion to the cat reporter gene in the multicopy vector pET2 is shown by open bars; activity measured after integration of the promoter fragment with the vector pRIM2 into the C. glutamicum chromosome is shown by solid bars. CAT activity is expressed relative to that measured with the WT promoter. Standard deviations (SD) from three independent measurements are indicated with error bars. (A) Mutations within the −10 hexamer; (B) mutations outside of the −10 hexamer.