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. 2023 May 26;6(3):157–169. doi: 10.1093/abt/tbad009

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© The Author(s) 2023. Published by Oxford University Press on behalf of Antibody Therapeutics. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.

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Enrichment and screen of cells displaying antibodies with cross-reactivity. (A) The top 0.2% and top 0.7% double-positive cells from both sublibraries were sorted to obtain single cells after staining by hFc-hC3d conjugated to Alexa Fluor 488 and hFc-mC3d conjugated to Alexa Fluor 647 in presence of 10% PNHCS. (B) Single-cell clones with binding activity to both hC3d and mC3d were verified by dual staining with hFc-hC3d conjugated to Alexa Fluor 488 and hFc-mC3d conjugated to Alexa Fluor 647 in presence of 10% PNHCS.