TABLE 1.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Description | Reference and/or source |
|---|---|---|
| E. coli strains | ||
| DH5α | F− φ80dlacZΔM15 Δ(lacZYA-argF)U169 endA1 recA1 hsdR17 (rK− mK+) deoR thi-1 supE44 gyrA96 relA1 λ− | 1 |
| AB1133 | K-12 thr-1 leuB6 Δ(gpt-proA)66 hisG4 argE3 thi-1 rfbD1 lacY1 ara-14 galK2 xyl-5 mtl-1 mgl-51 rpsL31 kdgK51 supE44 | 7 |
| 21546 | AB1133 with rff::Tn10-46 | 7 |
| 21566 | AB1133 with rff::Tn10-66 | 7 |
| B. fragilis strains | ||
| NCTC 9343 | Type strain, appendix abscess | 5, ATCCa |
| 638R (TM4000) | Clinical | 12 |
| 2-42 | 638R PS B mutant with transposon insertion in wcg locus | 2 |
| Plasmids | ||
| pHC79 | Cosmid vector; Apr | 4 |
| pUC19 | Cloning vector; Apr | New England Biolabs |
| pLEC6.1 | 900-bp EcoRI-HindIII fragment (B. fragilis DNA) cloned in pBluescript; Apr | 2 |
| pMJC10 | 638R gene bank clone including orf3-wcgE cloned in pHC79; Apr | This study |
| pLEC17 | 638R gene bank clone including wcgM-orf7 cloned in pHC79; Apr | This study |
| pLEC18 | 638R gene bank clone including wcgJ-orf7 cloned in pHC79; Apr | This study |
| pLEC19 | 638R gene bank clone including wcgF-orf7 cloned in pHC79; Apr | This study |
| pWCGC | 1,424-bp PCR amplicon containing wcgC cloned in pUC19; Apr | This study |
| pWCGD | 1,518-bp PCR amplicon containing wcgD cloned in pUC19; Apr | This study |
| pWCGCD | 2,711-bp PCR amplicon containing wcgC-wcgD cloned in pUC19; Apr | This study |
a
ATCC, American Type Culture Collection.