Fig. 5.

In vivo antitumor efficacy study with a schematic diagram on the workflow. Antitumor efficacy of IYIY-DNP conjugates and DNP in b TrkC + 4T1 and c TrkC- 67NR tumor-bearing mice. d Antitumor efficacy of IYIY-DNP in 4T1 and 67NR tumor-bearing mice. e Percentage of tumor reduction based on area under the tumor growth curve of IYIY-DNP and DNP in 4T1 and 67NR tumor-bearing mice, compared to respective immunized control mice. Arrow indicated the treatment given. The graph showed the mean ± SEM of tumor volume, n = 6. *p < 0.05 for IYIY-DNP- vs DNP-treated immunized mice, # p < 0.05 for IYIY-DNP vs immunized control saline, using nonparametric Mann–Whitney U-test. †p < 0.05 for 4T1 IYIY-DNP vs 67NR IYIY-DNP, using nonparametric Mann–Whitney U-test