TABLE 1.

Substrate inhibition of phthalate uptake by E. coli JM109 expressing ophD from B. cepacia 17616

Competing substratea	% Inhibition of phthalate uptakeb
Phthalate	91 ± 1
4-Hydroxyphthalate	86 ± 2
4-Chlorophthalate	66 ± 4
4-Methylphthalate	32 ± 4
Cinchomeronate	27 ± 6

^aThe concentrations of phthalate and competing substrate were 50 μM and 1 mM, respectively. 

^bThe following compounds inhibited phthalate uptake less than 10%: 4-nitrophthalate, 4-sulfophthalate, 3-nitrophthalate, quinolinate, o-chlorobenzoate, salicylate, benzoate, p-hydroxybenzoate, p-hydroxyphenylacetate, maleic acid, and tartrate. Values are averages from three to six experiments ± standard deviations.