Fig 3. Complex I and complex II-linked mitochondrial oxygen consumption of D. melanogaster frozen at -20°C and -80 °C.

The specific oxygen flux (pmol/(s*mL)) of D. melanogaster was assessed in samples that had been frozen at either -20°C (black) or -80°C (grey) in response to stepwise titrations of NADH (N), succinate (S), and rotenone (R), followed by antimycin A for background correction. D melanogaster -20°C (N = 3), D. melanogaster -80°C (N = 6). Error bars = SEM, * p < 0.05.