Table 2. Specific flux values of oxygen consumption from previously frozen D. melanogaster.
Mean specific flux values (pmol/(s*mL)) in homogenates of D. melanogaster previously frozen at -20°C and -80°C, in response to sequential titrations of substrates (NADH, succinate) and inhibitors (rotenone). D melanogaster -20°C (N = 3), D. melanogaster -80°C (N = 6), (SEM).
| Specific flux of substrate and inhibitor titrations (pmol/(s*mL)) | D. melanogaster | |
|---|---|---|
| -20 | -80 | |
| Routine | -0.745 (0.499) | -1.658 (0.310) |
| NADH | 1.847 (1.928) | 42.19 (10.176) |
| NADH, succinate | 2.334 (2.679) | 15.26 (2.915) |
| NADH, succinate, rotenone | 2.825 (2.608) | 15.48 (3.205) |