FIG. 1.

HPLC analysis of spore PG muropeptides. PG purified from spores produced by strains PS832 (wild type) (A), PS2066 (ΔdacB) (B), PS2307 (cwlD::Cm) (C), and PS2422 (cwlD::CmΔdacB) (D) was muramidase digested and separated by using a methanol gradient as previously described (14). Peaks are numbered as previously described (14, 15), and the structures of the muropeptides are given in Table 1. Peaks labeled B are buffer components seen in control samples. Peaks labeled X are reduction products of muramic-δ-lactam-containing muropeptides; corrections for this reduction were performed in calculation of PG structural parameters (14). No additional peaks were observed upon longer elution of the samples in panels C and D.