TABLE 1.
Muropeptide peaks derived from spore PG
Peaka | Structureb | Reference for structure | Amino acids released during hydrazinolysis |
---|---|---|---|
1 | DS-TriP | 14 | Dpm |
1A | DS-TriP-Gly | 15 | Gly |
2 | DS-Ala | 14 | Ala |
3 | DS-TP | 14 | Ala |
3A | DS-TP-Gly | Table 2 | Gly, Ala |
3B | DS-TP-Ala | Table 2 | Ala |
4 | DS-TP-TP | 14 | |
5 | TS-TP (open lactam) | 1, 14 | |
6 | TS-TP (reduced lactam) | 14 | |
7 | TS-Ala (reduced lactam) | 14 | |
7A | DS-TriP-Gly-DS-TP | 15 | Gly |
8 | DS-TriP-DS-TP | 14 | Dpm |
8A | DS-TP-Gly-DS-TP | Table 2 | Gly |
9 | DS-TP-DS-TP | 14 | Ala |
9A | DS-TP-Ala-DS-TP | Table 2 | Ala |
10 | TS-TP | 14 | |
11 | TS-TP-TP | 14 | |
12 | DS-TP-TS-TP (reduced lactam) | 14 | |
13 | TS-Ala | 14 | |
13A | DS-TP-DS-TP-DS-TP | 15 | |
13B | DS-TP-DS-TP-DS-TP-(Ala) | Table 2 | |
13C | DS-TP-DS-TP-DS-TP-DS-TP | Table 2 | |
14 | DS-TP-TS-TP | 14 | |
17 | TS-TP-TS-TP | 14 | |
18 | HS-TP | 14 | |
19 | HS-Ala | 14 | |
20 | TS-TP-HS-TP | 14 |
Peaks are numbered as shown in Fig. 1.
Abbreviations: HS, hexasaccharide; TS, tetrasaccharide. Where multiple saccharide moieties are indicated they are cross-linked via their peptide side chains with the first peptide providing the acceptor Dpm. The placement of an Ala residue in parentheses in muropeptide 13B indicates that this residue could be on either the first or second acceptor peptide.