TABLE 3.
Spore PG structural parametersa
| Strain (genotype) | % of muramic acid with side chain ofb:
|
% Peptides with Gly | % of muramic acid with a cross-linked peptidec | ||||
|---|---|---|---|---|---|---|---|
| Lactam | l-Ala | TP | TriP | TP-Ala | |||
| PS832 (wild type) | 50 | 24 ± 2 | 25 ± 2 | 2.1 ± 0.8 | 0 | 0 | 3.5 ± 0.6 |
| PS2066 (ΔdacB) | 46 ± 1 | 12 | 43 ± 2 | 2.2 ± 0.1 | 0 | 0 | 16.5 ± 1.3 (13.7) |
| PS2307 (cwlD::Cm) | 0 | 33 ± 1 | 58 ± 1 | 6.4 ± 0.4 | 1.2 ± 0.1 | 6.3 ± 0.7 | 7.9 ± 0.3 |
| PS2422 (cwlD::Cm ΔdacB) | 0 | 13 ± 3 | 80 ± 3 | 4.8 ± 0.4 | 2.4 ± 0.2 | 3.9 ± 1.0 | 32.4 ± 3.0 |
a
Data are averages of two (PS832, PS2066, and PS2307) or three (PS2422) analyses with errors of 1 standard deviation.
b
Lactam, muramic-δ-lactam. The values given for TP and TriP are the sum of TP and TP-Gly and the sum of TriP and TriP-Gly, respectively.
c
The value in parentheses is for effective cross-links; the remaining (ineffective) cross-links involved peptides which had been cleaved from the muramic acid (14). If no value is given in parentheses then there were not enough ineffective cross-links to alter the value.