Figure 2. Pcdhgs function in primary sensory neurons for normal tactile behaviors and sensorimotor integration.

(A) IHC images of DRG and spinal cord showing the GFP fused Pcdhg proteins in wildtype (negative control), Pcdhg^fcon3/fcon3 (positive control) and Advillin^Cre;Pcdhg^fcon3/fcon3 mice. IB4 labels lamina IIi nonpeptidergic nociceptors in the DRG.

(B) Diagram for the tactile PPI behavior assay.

(C) Percentage of inhibition of startle response to 125 dB noise when the startle noise is preceded by a light air puff of 0.9 PSI (500 ms ISI). Student’s unpaired t test.

(D) Quantification of the average movement of the back in response to 1.0 PSI air puff applied to the back hairy skin of P5 control and Advillin^Cre;Pcdhg^fcon3/fcon3 pups. Student’s unpaired t test.

(E and F) Von Frey thresholds (E) and response rates (F) for littermate control and Advillin^Cre;Pcdhg^fcon3/fcon3 mice. Two-way ANOVA. Fisher's LSD post hoc test.

(G) Diagram for the rough floor aversion assay.

(H) Percentages of time spent on rough side for littermate control and Advillin^Cre;Pcdhg^fcon3/fcon3 mice. Student’s unpaired t test.

(I) Distance traveled in an open field test. Student’s unpaired t test.

(J) Example images of the balance beam test.

(K and L) Total time it took for each animal to cross the beam (K) and average number of slips per trial (L) for littermate control and Advillin^Cre;Pcdhg^fcon3/fcon3 mice. Student’s unpaired t test.

Each dot represents an animal. ns, not significant; *p < 0.05; **p < 0.01; ***p < 0.001.