FIG. 2.

Reaction of hMYH and E. coli MutY (EcMY) with different mispair-containing 96-mer templates. Reactions were carried out for 3 h at 37°C; 3 μl of the protein preparation described in the legend to Fig. 1 and 6 ng of E. coli MutY protein were used for all templates. E. coli MutY was purified as described previously (6). The asterisks each indicate an A from a radiolabelled strand. s, substrate; p, product.