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. 2023 Jul 11;12:e81011. doi: 10.7554/eLife.81011

Figure 8. Motor skill learning defects of S421D mice are rescued by KIF1A silencing in vivo.

Figure 8.

(A) Schematic of the experimental procedure consisting in bilateral stereotaxic injections in the mouse brain followed 3 weeks later by the accelerating rotarod protocol over 8 days. (B) Mean time to fall off the rotarod per day over 8 days. Two-way ANOVA comparing the four conditions showed significant differences between genotypes and silencing conditions (****p<0.0001). Holm-Sidak’s post hoc analysis revealed significant differences between wild-type (WT) sh-Scr and HTT-SD sh-Scr mice at day 4 and day 8 (*p<0.05). (C) Mean time to fall off the rotarod the first day (*p<0.01) (left) and the last day (ns: non-significant, right), per sessions. Holm-Sidak’s post hoc analysis revealed significant differences between HTT-SD sh-Scr and HTT-SD sh-Kif1a mice during the first day (*p<0.05). At least three cohorts containing 12 WT sh-Scr, 11 WT sh-Kif1a, 10 HTT-SD sh-Scr, and 12 HTT-SD sh-Kif1a 3-month-old littermate male mice were analyzed.

Figure 8—source data 1. Data analyzed for accelerating rotarod over 8 days.
Figure 8—source data 2. Data analyzed for accelerating rotarod during day 1.
Figure 8—source data 3. Data analyzed for accelerating rotarod during day 8.