Figure 5.

Assessment of lateral scoliosis among all fish, male and female, in the tracking experiment. (A) Visualization of the scoliosis score. The scoliosis score is the sum of curvature measured at angle positions on the centerline at rest (see methods). To demonstrate how this score relates to body curvature, frames were taken from representative assays identified using k-medoids clustering of scoliosis scores of all assays. (B) Pairwise comparison of scoliosis scores between weeks by Spearman’s rank correlation. Scoliosis scores correlate weakly between control and 1 wpi to other assays. Scores are most strongly correlated from 2 wpi onward. (C) A demonstration that scoliosis ranks stabilize after 2 wpi. Scoliosis trend lines for individual fish are plotted over all assays (x-axis), colored by rank at 1, 2, 3, and 8 wpi (top-left to bottom-right). Fish scoliosis trend lines are colored on a rainbow scale from red (most severe) to violet (least), and colored gray if the fish did not survive to the ranked week. This figure includes 60, 58, 58, 58, 56, 56, 55, 54, 54 fish at control, 1, 2, 3, 4, 5, 6, 7, and 8 wpi assays, respectively.