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. 2023 Jul 10;16:1155754. doi: 10.3389/fnmol.2023.1155754

Figure 7.

Figure 7

Predicting 8 wpi regeneration outcomes using measurements taken by 2 wpi. (A) Tracking experiment: swim distance versus rostral compensation was averaged between 1 and 2 wpi. Dots represent individual fish and were colored based on the extent of glial bridging measured at 8 wpi for each fish. Fish that would develop larger glial bridges tended to swim further and exhibit less rostral compensation, in lower right side of each graph. Each fish was assigned a prediction score by adding its rank according to average swim distance to its rank according to average negative rostral compensation, relative to its sex group (Predicted poorly n = 30; predicted well n = 28). (B) Tracking experiment: 8 wpi glial bridging and axon regrowth outcomes for groups ranked high in swim distance and low in rostral compensation between 1 and 2 wpi (“high”) compared to those ranked low in swim distance and high in rostral compensation between 1 and 2 wpi (“low”) (Predicted poorly n = 27; predicted well n = 27). (C) Schematic overview of the prediction experiment. A total of 60 adult fish (30 males and 30 females) were housed in male–female pairs to track their identities until low/high regeneration predictions were assigned, then housed in prediction groups. At 8 wpi individuals were separated again to correlate final measurements from the endurance and behavior assays with cellular regeneration. Behavior was assayed before injury, 1 wpi, 2 wpi, and 8 wpi. Predictions were made, as described, after the 2 wpi behavior assay (Predicted poorly n = 30; predicted well n = 28). (D) Prediction experiment: swim distance versus rostral compensation was averaged between 1 and 2 wpi. Dots represent individual fish and were colored based on the predicted outcome. (E) Prediction experiment: 8 wpi glial bridging and axon regrowth outcomes for each prediction group (Predicted poorly n = 30; predicted well n = 26). Statistical significance in panels B and E was determined by Student’s t-test. p-values represent comparisons between prediction groups. ***p < 0.001; **p < 0.01; *p < 0.05; ns, p > 0.05.