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. 2023 Jul 10;17:1207540. doi: 10.3389/fncel.2023.1207540

Figure 3.

Figure 3

PolySia DP24–30 improves remyelination in a Siglec-E-dependent manner. OSCs from Siglec-E wildtype and knockout mice (Siglece+/+, Siglece−/−) were treated as indicated, and fixed at 14 DIV (for details, see Figure 1). (A, B) Representative images of MOG staining. Scale bars, 100 μm. (C) Morphometric evaluation. Data represent individual values and means ± SEM of MOG-positive areas in OSCs derived from n = 11–18 animals per group. Per animal eight OSCs were prepared, and two OSCs were assigned to each of the treatment groups. Per OSC, 3–4 frames of 350x 350 μm, each representing a different cerebellar lobule, were acquired and evaluated by morphometric determination of the MOG-positive area in percent of the total area. The two-way ANOVA revealed significant differences, and Tukey's post-hoc tests were applied. Significant differences within each genotype group and for selected comparisons between genotypes are indicated. Asterisks assigned to a single treatment group indicate significant differences against the untreated control of the same genotype (*P < 0.05; ****P < 0.0001).