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. 2023 Jul 10;17:1207540. doi: 10.3389/fncel.2023.1207540

Figure 4.

Figure 4

PolySia DP24–30 inhibits NO production of OSCs in a Siglec-E-dependent manner. NO production was determined by nitrite detection (Griess assay) in supernatants of OSCs treated with LPC and polySia DP8–14 or polySia DP24–30 as indicated. Each well contained four OSCs from two different animals, and supernatants were collected at 14DIV. Data represent individual values and means ± SEM of n = 8–10 values per group normalized to the level of untreated Siglece+/+ controls. To meet the assumption of normal distribution, the ROUT method with a false discovery rate of Q = 1% was used to eliminate four outliers (one in Siglece +/+, LPC, two in Siglece −/−, DP8–14, and one in Siglece −/− DP24–30). The two-way ANOVA revealed significant differences, and Tukey's post-hoc tests were applied. Significant differences within each genotype group and for selected comparisons between genotypes are indicated. Asterisks assigned to a single treatment group indicate significant differences against the untreated control of the same genotype (**P < 0.01; ***P < 0.001; ****P < 0.0001).