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Representative schematic of the high throughput flow cytometry system
A total of 5 μL of antibodies diluted 1:200 in PBS were added to each well containing 30 μL of cells (50,00 cells/well). This flow cytometry system captures the total events or cells per well in addition to the antibody activity, which measures markers including the surface receptors Ox40, CD80/86, and HLA-DR. The flow cytometry approach also identifies B cells, T cells, and monocytes.
