Antibody cocktail and AlphaLISA assay materials

Reagent	Final concentration	Amount
Antibody Cocktail.	Each Antibody combined and diluted 1:200 with dPBS by Volume.	∗
AlphaLISA Acceptor Bead Cocktail.	5 mg/mL Acceptor Beads and 500 nM Biotinylated Antibody combined 1:100 in 1X AlphaLISA buffer by volume	∗
AlphaLISA Donor Bead Cocktail.	5 mg/mL Donor Beads diluted 1:125 in 1X AlphaLISA buffer by volume.	∗

∗Volume can be amended based on screening throughput using the concentration scales indicated.