REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
CD134 (OX40) Antibody, anti-human, APC, REAfinity™ (Clone- REA621)	Miltenyi Biotec	Order Number: 130-122-227
CD80 Antibody, anti-human, PE, REAfinity™ (Clone- REA661). Diluted 1:200 with dPBS by volume.	Miltenyi Biotec	Order Number: 130-123-253
CD19 Antibody, anti-human, VioBlue, REAfinity™ (Clone- REA675). Diluted 1:200 with dPBS by volume.	Miltenyi Biotec	Order Number: 130-120-031
HLA-DR Antibody, anti-human, FITC, REAfinity™ (Clone- REA805). Diluted 1:200 with dPBS by volume.	Miltenyi Biotec	Order Number: 130-111-788
CD86 Antibody, anti-human, PE, REAfinity™ (Clone-REA968). Diluted 1:200 with dPBS by volume.	Miltenyi Biotec	Order Number: 130-116-160
Chemicals, peptides, and recombinant proteins
Dimethyl sulfoxide ACS	MP Biomedicals	Catalog Number: 191418
Paraformaldehyde, 16% w/v aq. Soln., methanol free	Alfa Aesar	Stock Number: 43368
Sterile water for irrigation, USP, 100mL	Baxter International	SKU: 2F7114
Reagent alcohol (denatured alcohol), 70% (v/v)	Ricca Chemical Company	Catalog Number: 2546.70-1
UltraPure™ distilled water	Invitrogen	Reference Number: 10977-015
Ficoll-PaqueTM sterile solution	Cytiva	Item Code: 17544203
Dulbecco’s Modified Eagle Medium	Gibco	Reference Number: 10566-106
Trypan blue stain (0.4%)	Gibco	Reference Number: 15250-061
Dulbecco’s phosphate buffered saline	Gibco	Reference Number: 14190-144
UltraPure™ 0.5 M EDTA, pH 8.0	Invitrogen	Reference Number: 15575-038
R848, 25 μM	Invivogen	TLRL-R848-5
CpG ODN-2395, 1 μM	Invivogen	TLRL-2395-5
Critical commercial assays
TNF-α AlphaLISA Detection Kit, 5,000 Assay Points	PerkinElmer	Product Number: AL208F
IFN-γ AlphaLISA Detection Kit, 5,000 Assay Points	PerkinElmer	Product Number: AL217F
IL-10 AlphaLISA Detection Kit, 5,000 Assay Points	PerkinElmer	Product Number: AL218F
Customized fluorometric bead-based array multiplex kit	Sigma-Aldrich	Product Code: HCYTA-60K
Software and algorithms
GraphPad Prism v8.3.1 for MacOS	GraphPad	https://www.graphpad.com
FlowJo v10	Becton Dickinson & Company	https://www.flowjo.com
Other
Corning 3656 384-Well Clear Round Bottom Non-Treated Plates	Corning	Product Number: 3656
AlphaPlate-384 Light Gray, Untreated Plates	PerkinElmer	Part Number: 6005350
Thermo Multidrop™ Combi Reagent Dispenser	Thermo Scientific	Catalog Number: 5840300