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CSK buffer
| Reagent | Final concentration | Amount |
|---|---|---|
| PIPES pH 6.8 (100 mM) | 10 mM | 5 mL |
| NaCl (2.5 M) | 100 mM | 2 mL |
| Sucrose (2 M) | 300 mM | 7.5 mL |
| MgCl2 (1 M) | 3 mM | 150 μL |
| cOmpleteTM, Mini, EDTA-free Protease Inhibitor Cocktail | N/A | 1 tablet |
| H2O | N/A | 35.35 mL |
| Total | N/A | 50 mL |
