Figure 1.

All major retinal cell types are captured by single-cell RNA-sequencing (scRNA-seq) and single-nucleus RNA-sequencing (snRNA-seq). A, Schematic summary of the study. Proliferative vitreoretinopathy (PVR) lesions were induced via lensectomy, pars plana vitrectomy, retinotomy, and extensive retinal detachment induction followed by retinal cryotherapy and intravitreal autologous platelet-rich plasma injection. At different time points, the lesions were dissected out, and cells were dissociated, or nuclei were isolated before profiling by scRNA-seq or snRNA-seq. B, C, Combined Uniform Manifold Approximation and Projection (UMAP) projection of all cells (left) or nuclei (right) profiled in this study. D, E, Examples of gene expression levels for selected cell type-specific genes for cells (left) or nuclei (right). RGC = retinal ganglion cell.