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. 2023 Jun 24;299(8):104951. doi: 10.1016/j.jbc.2023.104951

Figure 5.

Figure 5

Yeast A34 and A49 demonstrate co-dependent levels of expression.A, a double Δrpa34/Δrpa49 deletion strain was transformed with the indicated plasmids that express either Myc-tagged wild type A34, HA-tagged wild type A49, and/or their corresponding empty vectors. Equivalent amounts of yeast cells were serially diluted and spotted onto glucose complete (GC) medium lacking leucine and tryptophan and then grown at the indicated temperatures. B, whole-cell extracts from the yeast strains in A were prepared from liquid cultures and then analyzed by Western blot using the indicated epitope tag antibodies. C, the double Δrpa34/Δrpa49 deletion strain was co-transformed with a galactose inducible Myc-tagged A34 expression plasmid and an HA-tagged A49 expression vector. Cells were grown minimal media lacking Leucine and Tryptophan with glucose (GLU) as the carbon source or galactose (GAL). Whole extracts from these yeast strains were analyzed by Western blot using the indicated epitope tag antibodies. D, whole-cell extracts from the yeast strains in A were prepared from liquid cultures grown in the presence or absence of MG132 and then analyzed by Western blot. EV, empty vector.