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. 2023 Jun 24;299(8):104951. doi: 10.1016/j.jbc.2023.104951

Figure 6.

Figure 6

Inhibition of the IAA-induced proteasomal degradation of either AID-PAF49 or PAF53-AID rescues rDNA transcription.A, cells with AID-PAF49 were treated + 1 mM IAA in the presence or absence of 10 mM MG132 for 3 h. Whole-cell extracts were prepared and analyzed via Western blot. B, the same experiment from A was performed with cells containing PAF53-AID. C, cells with AID-tagged PAF49 were treated 1 mM IAA or vehicle in the presence or absence of 10 mM MG132 for 3 h. Following the 3 h treatment, cells were pulsed with 5-EU for 15 min. De novo synthesized RNA was visualized as described (red) against a DAPI background (blue) (94). Scale bar = 50 μm.