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. 2023 Jun 24;299(8):104951. doi: 10.1016/j.jbc.2023.104951

Figure 7.

Figure 7

Inhibition of rDNA transcription by depletion of PAF49 or PAF53 does not affect the steady-state levels of other Pol I subunits.A, cells expressing AID-PAF49 were treated with 1 mM IAA or vehicle for 3 h and were harvested and lysed in HEPES lysis buffer. The levels of multiple subunits of RNA Polymerase I (A194, A127, and A43) were determined by Western blot analysis. B, cells with PAF53-AID were treated with 1 mM IAA or vehicle for 3 h and were harvested and lysed in HEPES lysis buffer. The expression of the same Pol I subunits as in A were visualized by Western blot analysis. C, cells with AID-PAF49 were treated with IAA for 3 days. On each day, including day 0, cells were harvested and lysed as described previously. The expression of A194, PAF53, and A43 was determined by Western blot analysis. D, cells with PAF53-AID were treated with IAA for 3 days. On each day, including dy 0, cells were harvested and lysed as described previously. The expression of A194, PAF49, and A43 was determined by Western blot analysis.