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. 2023 Jul 10;13:1231986. doi: 10.3389/fonc.2023.1231986

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Copyright © 2023 Suzuki, Hamada, Ohkuma, Homma, Tsurui, Iriguchi, Ishiguro, Hirasawa, Ariizumi, Kubota, Horiike, Yoshimura, Wada, Yamochi and Tsunoda

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Liver biopsy tissue. HE staining: increased necrotic area after treatment. (A) Pretreatment. (B) After two courses. IHC: P16 was positive. Local lymphocyte infiltration was observed before the initiation of treatment. (C) P16 was positive (D) CD8⁺ T lymphocytes pretreatment. (E) CD8⁺ T lymphocytes after two courses. Lymphocyte accumulation was observed around the liver. HE, hematoxylin and eosin; IHC, immunohistochemistry.