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. 2023 Jul 6;5(7):1188–1203. doi: 10.1038/s42255-023-00834-7

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Extended Data Fig. 8 — a, Representative immunofluorescence images of human livers from lean (n=2) and individuals with obesity (n=2) imaged with Phenocycler, displaying region of interest highlighting five markers (DAPI, PanCK, SMA, CD3e, CD68) that are coloured according to the panel to the right. Scale bar, 100µm. b-c, Representative immunofluorescence image of tissue localization of resident (CD68⁺VSIG4⁺) and recruited (CD68⁺VSIG4^-) myeloid cells in human livers. Regions containing portal tracts (pt) and central vein (cv) are highlighted in the image by dashed white lines. Images are representative of 4 individuals. Scale bar, 100µm. d-e, Representative immunofluorescence image of tissue localization of recruited (CD68⁺S100A9⁺) myeloid cells in livers from lean and individuals with obesity. Regions containing portal tracts (pt) and central vein (cv) are highlighted in the image by dashed white circles. Scale bar, 100µm. Images are representative of 5 lean individuals and 4 individuals with obesity. LMs, liver myeloid cells; cv, central vein; pt, portal tract.