Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2023 Jul 6;5(7):1188–1203. doi: 10.1038/s42255-023-00834-7

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 3 — a, Experimental outline: liver biopsies and peripheral blood samples were collected before and 6 h after liver transplantation (n = 3 per group). Liver cells and PBMCs were then isolated and used for flow cytometric analysis to assess the proportion of recipient-derived and donor-derived cells. D-HLA, donor HLA; R-HLA, recipient HLA. b, Proportion of donor-derived or recipient-derived macrophages among all living CD45⁺CD68⁺HLA-DR⁺ myeloid cells after transplantation (n = 3 per group; blood donor versus recipient, P = 0.0024). c, Representative analysis of proportion of donor-derived cells as assessed by flow cytometric staining for donor-specific HLA. d, Proportion of LM subsets among all living CD45⁺CD68⁺HLA-DR⁺ myeloid cells in livers before (recipient, n = 3) and after (donor, n = 3) transplantation (LM4 before versus after, P < 0.0001). LM1 was defined as CD14⁺CD16⁺CD206⁺S100A9⁻, LM2 as CD14⁺CD16⁻CD206⁺S100A9⁻, LM3 as CD14⁺CD16⁺CD206−S100A9⁺ and LM4 as CD14⁺CD16⁻CD206⁻S100A9⁺. e, Representative images of lean human livers imaged using PhenoCycler, displaying the imaged tissue (left) and region of interest highlighting six markers (right; DAPI, PanCK, HLA-DR, CD68, CD163 and S100A9) that are colored according to the panel on the right. Regions containing portal tracts (pt) and central vein (cv) are highlighted in the image by dashed white lines. Images are representative of two individuals. Scale bar, 400 µm (left; entire tissue) and 100 µm (right; region of interest). f, Pseudospace plot visualizing the composition of resident and recruited myeloid cells sorted by tissue regions containing bile ducts (sorted to the right) in one lean donor. g, Representative images of livers of humans with obesity imaged using PhenoCycler. Images are representative of two individuals. Scale bar, 400 µm (left; entire tissue) and 100 µm (right; region of interest). h, Corresponding pseudospace plot. Data are presented as mean ± s.e.m. P values were calculated by two-way ANOVA with adjustment for multiple comparisons. **P < 0.01; ****P < 0.0001. Illustrations in a were partly created using components adapted from Servier Medical Art, provided by Servier, licensed under a Creative Commons Attribution 3.0 unported license.

Source data