Table 2.
Cassette position and materials/reagents list used in the radiosynthesis of [18F]FSPG via FASTlab™ module
| aCassette position (CP) | Reagents or consumables |
|---|---|
| 1 | Short tubing to 18O water collection vial |
| 2 | Potassium carbonate/kryptofix QMA elution solution, (11 mm Vial, 0.8 mL) |
| 3 | Left hand syringe (LHS), syringe 1 |
| 4 | QMA light SepPak cartridge, 1 pre-condition cartridge |
| 5 | Short tubing to QMA light SepPak cartridge at CP4 |
| 6 | 18F Inlet |
| 7 | Short tubing to reactor side port |
| 8 | Short tubing to reactor center port |
| 9 | 1M H2SO4, long tubing connected to external 4.0 mL 1M H2SO4 vial |
| 10 | bPhosphate buffered saline (PBS), long tubing connected to external 20 mL PBS vial |
| 11 | Middle syringe (MS), syringe 2 |
| 12 | FSPG precursor, (11 mm Vial, 6 -12 mg dissolved in 1.3 mL anhydrous acetonitrile) |
| 13 | Unused |
| 14 | 1M H2SO4, (13 mm Vial, 2.2 mL) |
| 15 | Water Spike/water bag, 100 mL SWI bag |
| 16 | 4M NaOH (13 mm Vial, 1.7 mL) |
| 17 | Short tubing to Oasis MCX cartridge at CP18 |
| 18 | Oasis® MCX cartridge, 1 cartridge |
| 19 | Short tubing to Oasis MCX cartridge at CP20 |
| 20 | Oasis® MCX cartridge, 1 cartridge |
| 21 | Short tubing to Alumina N plus long SepPak cartridge at CP22 |
| 22 | Alumina N plus long SepPak cartridge, 1 cartridge |
| 23 | Connecting tube to Superclean™ ENVI-Carb™ packed column, 1 set |
| 24 | Right hand syringe (RHS), syringe 3 |
| 25 | Long tubing to reactor side port |
| 26 | Final Product Vial, 1 vial |