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. 2023 Jul 24;14:4454. doi: 10.1038/s41467-023-40126-y

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© The Author(s) 2023, corrected publication 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — a Viral titer of rVSVs expressing G, ANDV Gn/Gc, or SNV Gn/Gc in human or mouse primary lung endothelial cells. Means ± SD: n = 6 wells of infected cells examined over three independent experiments. b Surface expression of endogenous PCDH1 in primary mouse lung microvascular endothelial cells (MLMECs) used in (a). Cells were immunostained with PCDH1-specific monoclonal antibody (mAb) 3305 or a negative control mAb (Ctrl.) Scale bar, 20 µm. c Infectivity of rVSVs bearing HTNV, ANDV, or SNV Gn/Gc in primary MLMECs expressing flag-tagged, wild-type (WT) or mouse-variant (F83L) human PCDH1. rVSV infectivities are expressed as fold change relative to that in non-complemented cells (set to one). Means ± SD: n = 35 wells of infected cells examined over three independent experiments. d Cells described in (c) were immunostained with an anti-flag antibody to detect total human PCDH1 expression in transfected MLMECs. Scale bar, 20 µm. e Alignment of human PCDH1-EC1 amino acid sequences with a selection of rodent and primate species. All of the residues within EC1 which deviate from the consensus are shown and highlighted. Residues that specifically deviate from human EC1 are indicated [green, experimentally tested SNV-susceptible hosts; purple, experimentally unknown; orange, residues in EC1 that are different between human and other species with no known link to susceptibility]. Alignments generated by Clustal Omega. f The capacity of U2OS PCDH1-KO cells expressing the indicated PCDH1 variants to support hantavirus Gn/Gc-dependent entry. Cells were exposed to rVSVs bearing the indicated Gn/Gc proteins or authentic ANDV, SNV, or HTNV. “None” indicates no PCDH1 expression. The infectivity of each virus was normalized to that obtained in U2OS PCDH1-KO cells complemented with WT PCDH1. rVSV means ± SD: n = 8 wells of infected cells examined over three independent experiments. ANDV/SNV/HTNV means ± SD: one experiment examining n = 3 (ANDV and SNV) or n = 10 (HTNV) wells of infected cells. Infectious units (a) were compared by unpaired, two-tailed t test with Welch’s correction. Infectivities (c, f) were compared by one-way ANOVA with Dunnett’s correction for multiple comparisons; ns > 0.05, **P < 0.01. Source data are provided as a Source Data file.