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. 2023 Jul 24;14:4454. doi: 10.1038/s41467-023-40126-y

Fig. 6. Two key amino acids in PCDH1 mediate entry for SNV and ANDV.

Fig. 6

a Relative infectivity of rVSVs bearing ANDV, SNV, or HTNV Gn/Gc on U2OS PCDH1-KO cells complemented with WT or mutant PCDH1. The infectivity of each virus was normalized to that obtained in U2OS PCDH1-KO cells complemented with WT PCDH1. Means ± SD: n = 9 wells of infected cells examined over three independent experiments (rVSV-ANDV-Gn/Gc infection on U2OS PCDH1-KO cells complemented with V86A had n = 8). Infectivities were compared by one-way ANOVA with Dunnett’s test for multiple comparisons. b Relative infectivity of authentic ANDV, SNV, or HTNV on the cell lines described in (a). The infectivity of each virus was normalized to that obtained in U2OS PCDH1-KO cells complemented with WT PCDH1. Means ± SD: n = 9 infected wells were examined over three independent experiments (HTNV infection on U2OS PCDH1-KO cells complemented with D85A and F83A/D85A had n = 6 wells of infected cells examined over two independent experiments). For ANDV and SNV infection on the control cell line, U2OS PCDH1-KO cells complemented with WT, n = 12 (SNV) and n = 18 (ANDV) wells of infected cells were examined over four independent experiments. Infectivities were compared by one-way ANOVA with Dunnett’s test for multiple comparisons. Source data are provided as a Source Data file.