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. 2023 Jul 24;13:11908. doi: 10.1038/s41598-023-38952-7

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Gene expression profiling via RNA-seq and GO analyses. HSCs were sorted 24 h after peritoneal administration of 5 mg/kg 6-MP and were analyzed via RNA-seq. (A) Scatter plot comparing Nudt15^+/+ HSC (WT, N = 3) and Nudt15^R138C/R138C HSC (R138C, N = 3). (B) GO analysis. Differentially expressed genes (log₂FC >|1|, p < 0.05) were analyzed using Metascape (http://metascape.org). The top 20 GO terms are presented. (C) Change in expression of genes listed under cellular response to DNA damage stimulus. (D) Change in expression of genes listed under the cell cycle process.