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. 2023 Jul 24;14:4450. doi: 10.1038/s41467-023-39700-1

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — Solid white lines outline the touch neuron soma. Scale bar = 2 µm. a Ad2 touch neuron, DIC-GFP merge, strain expressing bzIs166[P_mec-4mCherry]; ifd-1(ok2404); bzSi3[P_mec-7GFP::IFD-1]. Image is representative of N > 40 neurons. b Ad2 touch neuron, DIC-GFP merge, strain expressing bzIs166[P_mec-4mCherry]; bzSi37[P_mec-7mNeonGreen::IFD-2]. Image is representative of N > 20 neurons with visible mNG expression for this strain. c Top: L4 larval stage ALM (Anterior Lateral Microtubule) neuron Bottom: Ad11 ALM neuron. Strain expresses bzIs166[P_mec-4mCherry]; bzSi3[P_mec-7GFP::IFD-1]. Image is representative of N > 50 neurons. d Adult touch neuron expressing bzEx279[P_mec-7GFP::IFD-1 P_mec-7RFP::IFD-2]. Image is representative of N > 20 neurons. e Adult touch neuron expressing bzIs166[P_mec-4mCherry]; bzEx265[P_mec-4TagBFP::AMAN-2]; bzSi3[P_mec-7GFP::IFD-1]. Image is representative of N > 20 neurons. f Adult touch neuron expressing bzIs3[P_mec-7GFP::IFD-1]; pwSi222[P_mec-7LMP-1::mScarlet]. Image is representative of N > 20 neurons. g Adult touch neuron expressing bzIs166[P_mec-4mCherry]; bzEx265[P_mec-4TagBFP::AMAN-2]; bzSi3[P_mec-7GFP::IFD-1]. Image is representative of N > 20 neurons. h Colocalization correlation of GFP::IFD-1 and RFP::IFD-2 signals (d) graphed as Pearson’s Coefficient of red and green channel; boxes indicate the coefficient range with a minimum (0.74), maximum (0.97), and mean (0.89); N = 10 neurons. i Colocalization correlation of GFP::IFD-1 and TagBFP::AMAN-2 signals (e) graphed as Pearson’s Coefficient of blue and green channel; boxes indicate the coefficient range, with a minimum (0.34), maximum (0.62), and mean (0.50); N = 10 neurons. j Colocalization correlation of GFP::IFD-1 and LMP-1::mSc signals (f) graphed as Pearson’s Coefficient of red and green channel; boxes indicate the coefficient range, with a minimum (0.05), maximum (0.47), and mean is (0.42); N = 10 neurons. k Colocalization correlation of RFP::IFD-2 and mitoROGFP (g) graphed as Pearson’s Coefficient of red and green channel; boxes indicate the coefficient range, with a minimum (0.11), maximum (0.39), and mean (0.26); N = 10 neurons.