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. 2023 Jul 24;14:4450. doi: 10.1038/s41467-023-39700-1

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — a–e White solid outline indicates the soma cell body and white dashed line outlines the nucleus; scale bar = 2 µm. Colocalization of N = 10 ALMs is graphed as Mander’s overlap coefficient of mScarlet channel over mNeonGreen channel, with boxes indicating the coefficient range. a Representative adult touch neuron expressing single copy bzSi38[P_mec-7mNG::UBQ-2]; bzSi34[P_mec-7mSc::IFD-1]; range minimum (0.65), maximum (0.94), and mean (0.79) coefficient. b Representative adult touch neuron expressing bzEx422[P_mec-7mNG::UBQ-2]; bzEx421[P_mec-7mSc::IFD-2]; range minimum (0.20), maximum (0.98), and mean (0.66) coefficient. c Representative adult touch neuron expressing bzSi39[P_mec-7HttQ74::mNG]; bzSi34[P_mec-7mSc::IFD-1]; range minimum (0.26), maximum (0.85), and mean (0.59) coefficient. Images selected were those in which mNG was visibly aggregated in the cell, as opposed to homogeneous dim cytosolic signal. d Representative adult touch neuron expressing bzSi40[P_mec-7mNG::HttQ74]; bzEx420[P_mec-7mSc::IFD-2] that displays visible mNG aggregation; range minimum (0.15), maximum (0.72), and mean (0.57) coefficient. e Representative adult touch neuron expressing igIs1[P_mec-7YFP P_mec-3HttQ128::CFP]; bzSi34[P_mec-7mSc::IFD-1] that displays visible CFP and mSc co-expression; range minimum (0.43), maximum (0.99), and mean (0.56) coefficient. f HttQ128::CFP is expressed from a high-copy-number integrated transgene array. The IFD compartment often does not appear to fully surround the HttQ128 signal. Top row: Adult touch neuron soma displaying the three main patterns of interaction we note in lines that express mScarlet::IFD-1 and HttQ128::CFP. (1) HttQ128 and IFD overlap entirely (white arrows). (2) HttQ128 and IFD display a bi-lobed globular interaction, where part of the bi-lobed structure has overlap of HttQ128 and IFD. (3) HttQ128::CFP signal fully encompasses the IFD signal. Bottom row: side view z-stack (0.2 µm) from the image in the top row.