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. 2023 Jul 24;6:771. doi: 10.1038/s42003-023-05148-8

Fig. 4. Analysis of ZFP296 binding sites by ZFP296 ChIP-seq.

Fig. 4

a Pie chart showing the distribution of ZFP296 ChIP-seq peaks across genomic regions. b Plot showing the distribution of ChIP-seq peaks relative to known TSS. c Gene ontology (GO) biological processes enriched in the genes located near ZFP296-binding loci. d Most abundant DNA sequence motifs identified in ZFP296 ChIP-seq peaks. Consensus DNA binding motifs were computationally identified in the retrieved sequences of the ZFP296 binding peaks. e Localization of ZFP296 to heterochromatin. KO #98 and Zfp296−/−(CAG-Flag-Zfp296) cells (Rescue) were stained with anti-Flag-tag antibody followed by Alexa Fluor-labeled second antibody. Cells were counterstained with DAPI and observed by confocal fluorescence microscopy. Scale bars, 2 μm.