Fig. 6. Effects of ZFP296 binding on the chromatin accessibility.

a, b Upset plots showing overlap of ATAC-seq peaks among WT, KO #98, and Rescue #22 ESCs. The ATAC-seq peaks non-overlapping and overlapping with ZFP296 binding peaks were analyzed separately. c, d Scatter plots showing comparison of ATAC-seq normalized read counts within each merged ATAC-seq peak between KO–WT (left panels), KO–Rescue (middle panels), and Rescue-WT (right panels). The merged peaks non-overlapping (c) and overlapping (d) with ZFP296 binding peaks were analyzed separately. Peaks near the genes that showed significant difference in expression (P < 0.05) were marked as follows: Red dots represent the peaks near the genes whose normalized read counts of RNA-seq were no less than 6 and more than 3-fold higher in ESCs on the X-axis than on the Y-axis for each panel; Blue dots represent the peaks near the genes whose normalized read counts of RNA-seq were no less than 6 and more than 3-fold higher in ESCs on the Y-axis than on the X-axis for each panel. Gene names were put on the red dots in the left and middle panels (d).