Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2023 Jul 24;6:771. doi: 10.1038/s42003-023-05148-8

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 7 — a Histone H3K9 methylation in the presence or absence of Zfp296 expression. Western blot analysis was performed for H3K9me1/2/3 and H3K27me3 using nuclear extracts from WT, KO #98, and Rescue #22 ESCs. The density of each band relative to that with anti-histone H3 antibody was measured. The level of each histone modification was expressed relative to that in WT ESCs. Data represent means ± SD of five (H3K9me1 and H3K27me3) or six (H3K9me2/3) independent experiments. ^*P < 0.05 vs. Rescue cells by Student’s t-test. Some of these western blot analyses are shown in the right panel. b Coimmunoprecipitation analysis of the interaction between ZFP296 and G9a (see Methods). Flag-ZFP296, its deletion mutants, or ZFP296-EGFP were coexpressed with Myc-G9a in 293 T cells. Nuclear extracts were immunoprecipitated with anti-Flag-tag antibody, followed by SDS-PAGE and western blotting. The membrane was treated with anti-Myc-tag antibody followed by anti-Flag-tag antibody treatment. c, d Coimmunoprecipitation analysis of the interaction between ZFP296 or its deletion mutant ΔZF4-6 and GLP or G9a. Nuclear extracts were immunoprecipitated with anti-Flag-tag or anti-Myc-tag antibody, followed by SDS-PAGE and western blotting. The membrane was treated with anti-Myc-tag antibody followed by anti-Flag-tag antibody treatment. Immunoprecipitation of Myc-G9a with anti-Myc-tag antibody appeared inefficient compared with that of Myc-GLP.