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. 2023 Jul 24;13:11923. doi: 10.1038/s41598-023-39076-8

Figure 2.

Figure 2

Single-cycle kinetics analysis of surface plasmon resonance (SPR) sensorgrams for binding analysis of the different purified protein complexes. (A, B) Binding to PDL1 of the mono-specific protein Du at concentrations of 0.2, 0.33, 0.55, 0.93, 1.54, 2.57, 4.29, 7.14, and 11.9 nM (A) and the bi-specific protein DuRan-Bis at concentrations of 50, 62, 78, 98, 122, 153, 192, 240, and 300 nM (B). (C, D) Binding to VEGF121 of the mono-specific protein Ran at concentrations of 2, 4, 8, 16, 32, 64, 128, 256 and 512 nM (C) and the bi-specific protein DuRan-Bis at concentrations of 2, 4, 8, 16, 32, 64, 128, 256 and 512 nM (D). (E) Binding of the bi-specific protein DuRan-Bis (254 nM) to immobilized VEGF, followed by its binding to PDL1 (500 nM). The data was fitted to a 1:1 Langmuir kinetic binding model to yield the KD value for each protein pair. RU—response unit.