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. 2023 Jul 24;13:11922. doi: 10.1038/s41598-023-39093-7

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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The knockdown of GGCT activates the RB protein by down-regulating c-Met. We established PC3 and A549 cells that stably overexpressed c-Met. (a) A Western blot analysis of c-Met, GGCT, pMEK Ser217/221, pERK Thr202/Tyr204, pRB Ser780, and their non-phosphorylated forms in control vector-transfected or c-Met-overexpressing-clone#1 of PC3 and A549 cells, treated with control siRNA or GGCT siRNA#2 for 72 h. Vinculin is shown as a loading control. All full-length blots are presented in Supplementary Fig. S6. (b) Fractions in the S phase of A549 cells at 48 h post-transfection with the indicated siRNAs were measured by a BrdU incorporation assay. (c) Fractions in the S phase of control vector-transfected or c-Met-overexpressing-clone#1 and A549 cells at 48 h post-transfection with control siRNA or GGCT siRNA#2 were measured by a BrdU incorporation assay. (d) The ratio of the population of S phase cells to si-Ctrl of each group (control vector or c-Met overexpression) of (c) are shown. A two-tailed Student’s t-test was used (N = 3 per group, **p < 0.01). The error bars represent the S. D.