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. 2023 Jul 24;13:11922. doi: 10.1038/s41598-023-39093-7

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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The knockdown of GGCT suppresses STAT3 phosphorylation and nuclear localization. (a) A Western blot analysis of c-Met, pSTAT3 Ser727, and STAT3 in PC3 and A549 cells treated with Stattic for 72 h (PC3) or 48 h (A549) at the indicated concentrations. Vinculin is shown as a loading control. All full-length blots are presented in Supplementary Fig. S7. (b) The expression of human-MET mRNA, as assessed by qRT-PCR, in PC3 and A549 cells at 72 or 48 h post-treatment with Stattic. A one-way ANOVA with Dunnett’s test was used (versus Stattic 0 μmol/L, N = 3 per group, **p < 0.01, ****p < 0.0001). The error bars represent the S. D. (c) A Western blot analysis of pSTAT3 Ser727, and STAT3 in PC3 and A549 cells treated with control siRNA or the indicated GGCT siRNAs for 72 h. Vinculin is shown as a loading control. All full-length blots are presented in Supplementary Fig. S7. (d) Proteins from PC3 and A549 cells treated with the indicated siRNAs for 72 h were fractionated into cytoplasmic and nuclear components. The protein expression of GGCT and STAT3 was analyzed by Western blotting. Lamin A/C and vinculin are shown as fractionation and loading controls. All full-length blots are presented in Supplementary Fig. S7.