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. 2023 Jul 25;14(7):462. doi: 10.1038/s41419-023-05992-w

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 7 — a Scatter plot of the differential expressions of genes in the two different tumor lesions comparing to the corresponding adjacent normal tissues in each of the four MPLC patients. The x-axis represents the average log2FC (avg_logFC) computed by comparing the tumor and normal tissues in the inferior lobe (TIVSNI) of one patient. The y-axis represents the average log2FC (avg_logFC) computed by comparing the tumor and normal tissues in the other diseased lobe (TOthersVSNOthers) of one patient. The point colors represent the significance type. b Summary of the number of different significant types of genes identified based on each patient. c Summary of the number of different significant types of genes identified based on each cell type and each patient. d Seven Genes showed expressional change in all four MPLCs patients in certain cell types. e Box plot of the seven gene expressions in normal and tumor tissues based on the TCGA-LUAD dataset (p-value: kruskal test). f Violin plot of the expressions of TNFRSF18 across T&NK cell subpopulations. g Violin plot of the expressions of TNFRSF18 across all samples. h Spatially featured plot of the expressions of TNFRSF18 in two samples of P1.