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. 2023 Jul 24;14:4441. doi: 10.1038/s41467-023-40176-2

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — a The abundance of OsSGS3a and OsSGS3b in the IP-MS samples from OsSGS3a-Flag transgenic plants and NIP grown in the growth chamber at 28 °C. b OsSGS3a interacts with OsSGS3b and the CC domain of OsSGS3a is essential for their interaction in yeast. The different domains of OsSGS3a protein are indicated. c Split luciferase complementation (SLC) confirmation of the OsSGS3a and OsSGS3a/OsSGS3b interactions in N. benthamiana. Fluorescence signal intensity is indicated. Relative luciferase activity of the indicated protein-protein interactions was measured, shown as box plots (n = 9, biologically independent samples). Box plots show the median (central line) and interquartile range (IQR; from the 25th to 75th percentile); whiskers extend to minimum and maximum values within 1.5 times the IQR. Lowercase letters indicate significant differences at P < 0.05, as determined by one-way ANOVA with the Tukey’s HSD post hoc test. Exact P values are provided in Supplementary Data 7. d Detection of OsSGS3a interaction with OsSGS3b by Co-immunoprecipitation (Co-IP) in rice. Source data are provided as a Source Data file (c, d). Experiments were repeated two times with similar results (b–d).