Fig. 2. TAA induced SNr neurons, but not cerebral cortex neurons, suffering an oxidative attack and mitochondrial dysfunction.
a SNr expression of LC3B, PINK1, UCP2, SOD1, and GPX1 from Sham and TAA, assayed by western blot. b Quantitative analysis of a. c SNr expression of UCP4 and UCP5 from Sham and TAA, assayed by western blot. d Quantitative analysis of c. e SNr expression of DRP1, p-DRP1(Ser616), MFN2, MFF, and FIS1 from Sham and TAA, assayed by western blot. f Quantitative analysis of e. g Schematic of a sagittal brain section and confocal images showing the highest bright GFP concentrated at OB, CC, CPu, SNr, CB, and BS from a GAD2-Mito-GFP transgenic mouse. (Bar = 1 mm). h, i Confocal images of mitochondrial GFP at SNr and its statistical results by MiNA analysis (n = 4 per group, Bar = 100 µm). j Cortex expression of UCP2, UCP4, UCP5, SOD1, and GPX1 from Sham and TAA, assayed by western blot. k Quantitative analysis of j. l Cortex expression of LC3B and PINK1 from Sham and TAA, assayed by western blot. m Quantitative analysis of l (n = 3 per group). n Cortex expression of DRP1, p-DRP1(Ser616), MFN2, MFF, and FIS1 from Sham and TAA, assayed by western blot. o Quantitative analysis of n. Data are presented as means ± SD. *P < 0.05, **P < 0.01vs Sham. n = 3 per group in western blotting. Two-tailed, unpaired, Student’s t-test for all data. OB olfactory bulb, CC cerebral cortex, CPu caudate putamen (striatum), CB cerebellum, BS brainstem, SNr substantia nigra pars reticulate. Source data are provided as a Source Data file.